PDBsum entry 7owc

Immune system

PDB id: 7owc

Contents

Protein chains

74 a.a.

95 a.a.

Waters ×179

PDB id:

7owc

Name:

Immune system

Title:

Structure of cyld cap-gly3 (467-565) bound to ub; orthorhobic space group

Structure:

Ubiquitin-60s ribosomal protein l40. Chain: a, c. Synonym: cep52,ubiquitin a-52 residue ribosomal protein fusion product 1. Engineered: yes. Deubiquitinating enzyme cyld. Chain: b, d. Synonym: ubiquitin carboxyl-terminal hydrolase cyld,ubiquitin thioesterase cyld,ubiquitin-specific-processing protease cyld.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: uba52, ubcep2. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: cyld. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.85Å R-factor: 0.208 R-free: 0.239

Authors:

P.R.Elliott,D.Komander

Key ref:

P.R.Elliott et al. (2021). Regulation of cyld activity and specificity by phosphorylation and ubiquitin-Binding cap-Gly domains. Cell rep, 37, 09777. PubMed id: 34610306

Date:

17-Jun-21 Release date: 20-Oct-21

Protein chains

P62987  (RL40_HUMAN) -  Ubiquitin-ribosomal protein eL40 fusion protein from Homo sapiens

Seq: 128 a.a.

Struc: 74 a.a.*

Protein chains

Q9NQC7  (CYLD_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase CYLD from Homo sapiens

Seq: 956 a.a.

Struc: 95 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: Chains A, C: E.C.?
• Enzyme class 2: Chains B, D: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.