PDBsum entry 7nu1

Ligase

PDB id: 7nu1

Contents

Protein chain

805 a.a.

Metals

_MG

_ZN

Waters ×115

PDB id:

7nu1

Name:

Ligase

Title:

Crystal structure of neisseria gonorrhoeae leurs e169g mutant

Structure:

Leucine--tRNA ligase. Chain: a. Fragment: leucyl-tRNA synthetase. Synonym: leucyl-tRNA synthetase,leurs. Engineered: yes. Mutation: yes

Source:

Neisseria gonorrhoeae. Organism_taxid: 485. Strain: nccp11945. Gene: leus, ngk_0009. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.51Å R-factor: 0.206 R-free: 0.262

Authors:

L.Pang,S.V.Strelkov,S.D.Weeks

Key ref:

L.Pang et al. Partitioning of the initial catalytic steps of leucyl synthetase is driven by an active site peptide-Plane. Commun biol, . PubMed id: 36038645

Date:

11-Mar-21 Release date: 31-Aug-22

Protein chain

B4RNT1  (SYL_NEIG2) -  Leucine--tRNA ligase from Neisseria gonorrhoeae (strain NCCP11945)

Seq: 876 a.a.

Struc: 805 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.6.1.1.4 - leucine--tRNA ligase.
• Reaction: tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site