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PDBsum entry 7mjh
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Viral protein
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PDB id
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7mjh
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References listed in PDB file
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Key reference
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Title
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Cryo-Electron microscopy structures of the n501y sars-Cov-2 spike protein in complex with ace2 and 2 potent neutralizing antibodies.
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Authors
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X.Zhu,
D.Mannar,
S.S.Srivastava,
A.M.Berezuk,
J.P.Demers,
J.W.Saville,
K.Leopold,
W.Li,
D.S.Dimitrov,
K.S.Tuttle,
S.Zhou,
S.Chittori,
S.Subramaniam.
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Ref.
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PLoS Biol, 2021,
19,
e3001237.
[DOI no: ]
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PubMed id
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Abstract
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The recently reported "UK variant" (B.1.1.7) of SARS-CoV-2 is thought
to be more infectious than previously circulating strains as a result of several
changes, including the N501Y mutation. We present a 2.9-Å resolution
cryo-electron microscopy (cryo-EM) structure of the complex between the ACE2
receptor and N501Y spike protein ectodomains that shows Y501 inserted into a
cavity at the binding interface near Y41 of ACE2. This additional interaction
provides a structural explanation for the increased ACE2 affinity of the N501Y
mutant, and likely contributes to its increased infectivity. However, this
mutation does not result in large structural changes, enabling important
neutralization epitopes to be retained in the spike receptor binding domain. We
confirmed this through biophysical assays and by determining cryo-EM structures
of spike protein ectodomains bound to 2 representative potent neutralizing
antibody fragments.
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