PDBsum entry 7ew2

Membrane protein

PDB id: 7ew2

Contents

Protein chains

226 a.a.

340 a.a.

61 a.a.

232 a.a.

290 a.a.

Ligands

J89

PDB id:

7ew2

Name:

Membrane protein

Title:

Cryo-em structure of pfty720-bound sphingosine 1-phosphate receptor 3 in complex with gi protein

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnb1. Gene: gng2. Gene: s1pr3, edg3.

Authors:

C.Zhao,W.Wang,H.L.Wang,Z.H.Shao

Key ref:

C.Zhao et al. Structural insights into sphingosine-1-Phosphate reco and ligand selectivity of s1pr3-Gi signaling complexe. Cell res, . PubMed id: 34545189

Date:

24-May-21 Release date: 29-Sep-21

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 226 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 340 a.a.*

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 61 a.a.

Protein chain

No UniProt id for this chain

Struc: 232 a.a.

Protein chain

Q99500  (S1PR3_HUMAN) -  Sphingosine 1-phosphate receptor 3 from Homo sapiens

Seq: 378 a.a.

Struc: 290 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????