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PDBsum entry 7d7c
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Transcription
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PDB id
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7d7c
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Contents |
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219 a.a.
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1316 a.a.
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1335 a.a.
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137 a.a.
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References listed in PDB file
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Key reference
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Title
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Transcription activation by a sliding clamp.
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Authors
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J.Shi,
A.Wen,
S.Jin,
B.Gao,
Y.Huang,
Y.Feng.
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Ref.
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Nat Commun, 2021,
12,
1131.
[DOI no: ]
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PubMed id
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Abstract
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Transcription activation of bacteriophage T4 late genes is accomplished by a
transcription activation complex containing RNA polymerase (RNAP), the promoter
specificity factor gp55, the coactivator gp33, and a universal component of
cellular DNA replication, the sliding clamp gp45. Although genetic and
biochemical studies have elucidated many aspects of T4 late gene transcription,
no precise structure of the transcription machinery in the process is available.
Here, we report the cryo-EM structures of a gp55-dependent RNAP-promoter open
complex and an intact gp45-dependent transcription activation complex. The
structures reveal the interactions between gp55 and the promoter DNA that
mediate the recognition of T4 late promoters. In addition to the σR2 homology
domain, gp55 has a helix-loop-helix motif that chaperons the template-strand
single-stranded DNA of the transcription bubble. Gp33 contacts both RNAP and the
upstream double-stranded DNA. Gp45 encircles the DNA and tethers RNAP to it,
supporting the idea that gp45 switches the promoter search from
three-dimensional diffusion mode to one-dimensional scanning mode.
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