 |
PDBsum entry 7b07
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
|
|
|
|
|
|
|
|
|
DNA binding protein
|
PDB id
|
|
|
|
7b07
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
 |
|
 |
| Name: |
 |
DNA binding protein
|
 |
|
Title:
|
 |
Tgot_6g12 apo
|
|
Structure:
|
 |
DNA polymerase. Chain: a. Synonym: to pol. Engineered: yes
|
|
Source:
|
 |
Thermococcus gorgonarius. Organism_taxid: 71997. Gene: pol, pola. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008
|
|
Resolution:
|
 |
|
3.10Å
|
R-factor:
|
0.262
|
R-free:
|
0.265
|
|
|
Authors:
|
 |
C.Samson,P.Legrand,M.Tekpinar,J.Rozenski,M.Abramov,P.Holliger, V.Pinheiro,P.Herdewijn,M.Delarue
|
|
Key ref:
|
 |
C.Samson
et al.
(2020).
Structural studies of hna substrate specificity in mu of an archaeal DNA polymerase obtained by directed evolution..
Biomolecules,
10,
.
PubMed id:
|
 |
|
Date:
|
 |
|
18-Nov-20
|
Release date:
|
30-Dec-20
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
Headers
|
 |
|
|
References
|
|
|
|
|
|
|
P56689
(DPOL_THEGO) -
DNA polymerase from Thermococcus gorgonarius
|
|
|
|
Seq: Struc:
|
 |
 |
 |
773 a.a.
749 a.a.*
|
|
|
|
|
|
|
|
|
 |
 |
|
|
Key: |
 |
 |
Secondary structure |
 |
|
*
PDB and UniProt seqs differ
at 18 residue positions (black
crosses)
|
|
|
|
|
 |
|
|
 |
 |
 |
 |
Enzyme class:
|
 |
E.C.2.7.7.7
- DNA-directed Dna polymerase.
|
|
 |
 |
 |
 |
 |
Reaction:
|
 |
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
|
 |
 |
 |
 |
 |
DNA(n)
|
+
|
2'-deoxyribonucleoside 5'-triphosphate
|
=
|
DNA(n+1)
|
+
|
diphosphate
|
|
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
|
|
 |
');
}
}
 |