PDBsum entry 7xk8

Membrane protein

PDB id: 7xk8

Contents

Protein chains

26 a.a.

282 a.a.

225 a.a.

338 a.a.

53 a.a.

PDB id:

7xk8

Name:

Membrane protein

Title:

Cryo-em structure of the neuromedin u receptor 2 (nmur2) in complex with g protein and its endogeneous peptide-agonist nmu25

Structure:

Neuromedin-u-25. Chain: p. Synonym: nmu-25. Engineered: yes. Neuromedin-u receptor 2. Chain: r. Synonym: nmu-r2,g-protein coupled receptor fm-4,g-protein coupled receptor tgr-1. Engineered: yes.

Source:

Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Other_details: c-terminal amidation. Gene: nmur2, nmu2r, tgr1. Expressed in: insecta environmental sample. Expression_system_taxid: 2588572. Gene: gnai1.

Authors:

W.Zhao,Z.Wenru,W.Mu,L.Minmin,C.Shutian,T.Tingting,S.Gisela,W.Holger, B.Albert,Y.Cuiying,C.Xiaojing,S.Han,B.Wu,Q.Zhao

Key ref:

W.Zhao et al. (2022). Ligand recognition and activation of neuromedin u rec 2.. Nat commun, 13, 7955. PubMed id: 36575163

Date:

19-Apr-22 Release date: 22-Feb-23

Protein chain

P48645  (NMU_HUMAN) -  Neuromedin-U from Homo sapiens

Seq: 174 a.a.

Struc: 26 a.a.*

Protein chain

Q9GZQ4  (NMUR2_HUMAN) -  Neuromedin-U receptor 2 from Homo sapiens

Seq: 415 a.a.

Struc: 282 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 225 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 53 a.a.

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????