PDBsum entry 7vug

Membrane protein

PDB id: 7vug

Contents

Protein chains

218 a.a.

338 a.a.

57 a.a.

282 a.a.

232 a.a.

Ligands

7ZQ

PDB id:

7vug

Name:

Membrane protein

Title:

Cryo-em structure of a class a orphan gpcr in complex with gi

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnb1. Gene: gng2. Bacillus circulans, homo sapiens.

Authors:

Z.J.Liu,T.Hua,Y.L.Zhou,L.J.Wu

Key ref:

Y.Zhou et al. Molecular insights into ligand recognition and g prot coupling of the neuromodulatory orphan receptor gpr13. Cell res, . PubMed id: 34916631

Date:

02-Nov-21 Release date: 29-Dec-21

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 218 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 57 a.a.

Protein chain

P09850  (XYNA_NIACI) -  Endo-1,4-beta-xylanase from Niallia circulans

Seq: 213 a.a.

Struc: 282 a.a.*

Protein chain

Q6DWJ6  (GP139_HUMAN) -  Probable G-protein coupled receptor 139 from Homo sapiens

Seq: 353 a.a.

Struc: 282 a.a.*

Protein chain

No UniProt id for this chain

Struc: 232 a.a.

* PDB and UniProt seqs differ at 133 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 3: Chain R: E.C.3.2.1.8 - endo-1,4-beta-xylanase.
• Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.