PDBsum entry 7ld3

Signaling protein

PDB id: 7ld3

Contents

Protein chains

210 a.a.

288 a.a.

334 a.a.

56 a.a.

Ligands

ADN

XTD

Waters ×1

PDB id:

7ld3

Name:

Signaling protein

Title:

Cryo-em structure of the human adenosine a1 receptor-gi2-protein complex bound to its endogenous agonist and an allosteric ligand

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-2. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Chimera protein of muscarinic acetylcholine receptor m4 and adenosine receptor a1. Chain: r. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai2, gnai2b. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: chrm4, adora1. Gene: gnb1. Gene: gng2.

Authors:

C.J.Draper-Joyce,R.Danev,D.M.Thal,A.Christopoulos,A.Glukhova

Key ref:

C.J.Draper-Joyce et al. (2021). Positive allosteric mechanisms of adenosine a 1 receptor-Mediated analgesia.. Nature, 597, 571. PubMed id: 34497422

Date:

12-Jan-21 Release date: 08-Sep-21

Protein chain

P04899  (GNAI2_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-2 from Homo sapiens

Seq: 355 a.a.

Struc: 210 a.a.*

Protein chain

P08173  (ACM4_HUMAN) -  Muscarinic acetylcholine receptor M4 from Homo sapiens

Seq: 479 a.a.

Struc: 288 a.a.*

Protein chain

P30542  (AA1R_HUMAN) -  Adenosine receptor A1 from Homo sapiens

Seq: 326 a.a.

Struc: 288 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 334 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 56 a.a.

* PDB and UniProt seqs differ at 271 residue positions (black crosses)