PDBsum entry 7bw0

Membrane protein

PDB id: 7bw0

Contents

Protein chains

280 a.a.

225 a.a.

340 a.a.

58 a.a.

128 a.a.

PDB id:

7bw0

Name:

Membrane protein

Title:

Active human tgr5 complex with a synthetic agonist 23h

Structure:

Soluble cytochrome b562,g-protein coupled bile acid receptor 1. Chain: r. Synonym: cytochrome b-562,g-protein coupled receptor gpcr19,hgpcr19, membrane-type receptor for bile acids,m-bar,hbg37,bg37. Engineered: yes. Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(s) subunit alpha isoforms short.

Source:

Escherichia coli, homo sapiens. Human. Organism_taxid: 562, 9606. Gene: cybc, gpbar1, tgr5. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Homo sapiens. Organism_taxid: 9606. Gene: gnas, gnas1, gsp.

Authors:

G.Chen,X.K.Wang,Q.Chen,H.L.Hu,R.B.Ren

Key ref:

G.Chen et al. (2020). Cryo-EM structure of activated bile acids receptor TGR5 in complex with stimulatory G protein. Signal Transduct Target Ther, 5, 142. PubMed id: 32747649 DOI: 10.1038/s41392-020-00262-z

Date:

12-Apr-20 Release date: 02-Sep-20

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 280 a.a.*

Protein chain

Q8TDU6  (GPBAR_HUMAN) -  G-protein coupled bile acid receptor 1 from Homo sapiens

Seq: 330 a.a.

Struc: 280 a.a.

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 225 a.a.*

Protein chain

P54311  (GBB1_RAT) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Rattus norvegicus

Seq: 340 a.a.

Struc: 340 a.a.*

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 58 a.a.

Protein chain

No UniProt id for this chain

Struc: 128 a.a.

* PDB and UniProt seqs differ at 73 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains R, A, B, G: E.C.?

DOI no: 10.1038/s41392-020-00262-z

Signal Transduct Target Ther 5:142 (2020)

PubMed id: 32747649

Cryo-EM structure of activated bile acids receptor TGR5 in complex with stimulatory G protein.

G.Chen, X.Wang, Y.Ge, L.Ma, Q.Chen, H.Liu, Y.Du, R.D.Ye, H.Hu, R.Ren.

ABSTRACT

No abstract given.