PDBsum entry 6tfp

Transferase

PDB id: 6tfp

Contents

Protein chains

266 a.a.

Ligands

N6Z ×5

Metals

_NA ×2

Waters ×653

References listed in PDB file

Key reference

• Title: Discovery of lou064 (remibrutinib), A potent and highly selective covalent inhibitor of bruton'S tyrosine kinase.
• Authors: D.Angst, F.Gessier, P.Janser, A.Vulpetti, R.Wälchli, C.Beerli, A.Littlewood-Evans, J.Dawson, B.Nuesslein-Hildesheim, G.Wieczorek, S.Gutmann, C.Scheufler, A.Hinniger, A.Zimmerlin, E.G.Funhoff, R.Pulz, B.Cenni.
• Ref.: J Med Chem, 2020, 63, 5102-5118. [DOI no: 10.1021/acs.jmedchem.9b01916]
• PubMed id: 32083858

Abstract

Bruton's tyrosine kinase (BTK), a cytoplasmic tyrosine kinase, plays a central role in immunity and is considered an attractive target for treating autoimmune diseases. The use of currently marketed covalent BTK inhibitors is limited to oncology indications based on their suboptimal kinase selectivity. We describe the discovery and preclinical profile of LOU064 (remibrutinib, 25), a potent, highly selective covalent BTK inhibitor. LOU064 exhibits an exquisite kinase selectivity due to binding to an inactive conformation of BTK and has the potential for a best-in-class covalent BTK inhibitor for the treatment of autoimmune diseases. It demonstrates potent in vivo target occupancy with an EC₉₀ of 1.6 mg/kg and dose-dependent efficacy in rat collagen-induced arthritis. LOU064 is currently being tested in phase 2 clinical studies for chronic spontaneous urticaria and Sjoegren's syndrome.