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PDBsum entry 6rok
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PDB id:
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Hydrolase
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Title:
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The crystal structure of a complex between the llfpg protein, a thf- DNA and an inhibitor
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Structure:
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Formamidopyrimidine-DNA glycosylase. Chain: a. Synonym: fapy-DNA glycosylase,DNA-(apurinic or apyrimidinic site) lyase mutm,ap lyase mutm. Engineered: yes. DNA (5'-d( Cp Tp Cp Tp Tp Tp (3Dr)p Tp Tp Tp Cp Tp Cp G)- 3'). Chain: d. Engineered: yes.
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Source:
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Lactococcus lactis subsp. Cremoris. Organism_taxid: 1359. Gene: mutm, fpg, ncdo763_0992. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630
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Resolution:
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1.95Å
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R-factor:
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0.169
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R-free:
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0.196
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Authors:
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F.Coste,S.Goffinont,B.Castaing
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Key ref:
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C.Rieux
et al.
(2020).
Thiopurine derivative-Induced fpg/nei DNA glycosylase inhibition: structural, Dynamic and functional insigh.
Int j mol sci,
21,
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PubMed id:
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Date:
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13-May-19
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Release date:
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15-Apr-20
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PROCHECK
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Headers
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References
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P42371
(FPG_LACLC) -
Formamidopyrimidine-DNA glycosylase from Lactococcus lactis subsp. cremoris
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Seq:
Struc:
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273 a.a.
271 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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C-T-C-T-T-T-3DR-T-T-T-C-T-C-G
14 bases
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G-C-G-A-G-A-A-A-C-A-A-A-G-A
14 bases
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Enzyme class 1:
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E.C.3.2.2.23
- DNA-formamidopyrimidine glycosylase.
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Reaction:
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Hydrolysis of DNA containing ring-opened N(7)-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide.
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Enzyme class 2:
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E.C.4.2.99.18
- DNA-(apurinic or apyrimidinic site) lyase.
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Reaction:
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2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)- 2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho- 2'-deoxyribonucleoside-DNA + H+
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Links
