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PDBsum entry 6psr
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Transcription/DNA
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PDB id
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6psr
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Contents |
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230 a.a.
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219 a.a.
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1340 a.a.
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1345 a.a.
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75 a.a.
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548 a.a.
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73 a.a.
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72 a.a.
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References listed in PDB file
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Key reference
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Title
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Stepwise promoter melting by bacterial RNA polymerase.
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Authors
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J.Chen,
C.Chiu,
S.Gopalkrishnan,
A.Y.Chen,
P.D.B.Olinares,
R.M.Saecker,
J.T.Winkelman,
M.F.Maloney,
B.T.Chait,
W.Ross,
R.L.Gourse,
E.A.Campbell,
S.A.Darst.
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Ref.
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Mol Cell, 2020,
78,
275.
[DOI no: ]
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PubMed id
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Abstract
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Transcription initiation requires formation of the open promoter complex (RPo).
To generate RPo, RNA polymerase (RNAP) unwinds the DNA duplex to form the
transcription bubble and loads the DNA into the RNAP active site. RPo formation
is a multi-step process with transient intermediates of unknown structure. We
use single-particle cryoelectron microscopy to visualize seven intermediates
containing Escherichia coli RNAP with the transcription factor TraR en route to
forming RPo. The structures span the RPo formation pathway from initial
recognition of the duplex promoter in a closed complex to the final RPo. The
structures and supporting biochemical data define RNAP and promoter DNA
conformational changes that delineate steps on the pathway, including previously
undetected transient promoter-RNAP interactions that contribute to populating
the intermediates but do not occur in RPo. Our work provides a structural basis
for understanding RPo formation and its regulation, a major checkpoint in gene
expression throughout evolution.
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