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PDBsum entry 6obh
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Viral protein
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PDB id
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6obh
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References listed in PDB file
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Key reference
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Title
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Modular HIV-1 capsid assemblies reveal diverse host-Capsid recognition mechanisms.
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Authors
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B.J.Summers,
K.M.Digianantonio,
S.S.Smaga,
P.T.Huang,
K.Zhou,
E.E.Gerber,
W.Wang,
Y.Xiong.
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Ref.
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Cell Host Microbe, 2019,
26,
203.
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PubMed id
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Abstract
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The HIV-1 capsid is an ordered protein shell that houses the viral genome during
early infection. Its expansive surface consists of an ordered and interfacing
array of capsid protein hexamers and pentamers that are recognized by numerous
cellular proteins. Many of these proteins recognize specific, assembled capsid
interfaces not present in unassembled capsid subunits. We used
protein-engineering tools to capture diverse capsid assembly intermediates. We
built a repertoire of capsid assemblies (ranging from two to 42 capsid protein
molecules) that recreate the various surfaces in infectious capsids. These
assemblies reveal unique capsid-targeting mechanisms for each of the anti-HIV
factors, TRIMCyp, MxB, and TRIM5α, linked to inhibition of virus uncoating and
nuclear entry, as well as the HIV-1 cofactor FEZ1 that facilitates virus
intracellular trafficking. This capsid assembly repertoire enables elucidation
of capsid recognition modes by known capsid-interacting factors, identification
of new capsid-interacting factors, and potentially, development of
capsid-targeting therapeutics.
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