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PDBsum entry 6f08
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Peptide binding protein
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PDB id
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6f08
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PDB id:
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| Name: |
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Peptide binding protein
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Title:
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14-3-3 zeta in complex with the human son of sevenless homolog 1 (sos1)
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Structure:
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14-3-3 protein zeta/delta. Chain: a, b, i, j. Synonym: protein kinasE C inhibitor protein 1,kcip-1. Engineered: yes. Son of sevenless homolog 1. Chain: d, k, n, q. Synonym: sos-1. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: ywhaz. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 9606
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Resolution:
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1.90Å
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R-factor:
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0.213
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R-free:
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0.267
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Authors:
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A.Ballone,F.Centorrino,C.Ottmann,S.Guo,S.Leysen
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Key ref:
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A.Ballone
et al.
(2018).
Structural characterization of 14-3-3ζ in complex with the human Son of sevenless homolog 1 (SOS1).
J Struct Biol,
202,
210-215.
PubMed id:
DOI:
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Date:
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17-Nov-17
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Release date:
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14-Feb-18
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PROCHECK
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Headers
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References
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P63104
(1433Z_HUMAN) -
14-3-3 protein zeta/delta from Homo sapiens
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Seq:
Struc:
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245 a.a.
225 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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DOI no:
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J Struct Biol
202:210-215
(2018)
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PubMed id:
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Structural characterization of 14-3-3ζ in complex with the human Son of sevenless homolog 1 (SOS1).
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A.Ballone,
F.Centorrino,
M.Wolter,
C.Ottmann.
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ABSTRACT
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The deviant Ras activation machinery is found in approximately 30% of all human
cancers. SOS1 is an important protagonist of this pathway that plays a key-role
in aberrant cell proliferation and differentiation. Interaction of SOS1 with
14-3-3 proteins modulates SOS1 activity in Ras-MAPK signaling. In the present
study, we analyze the 14-3-3/SOS1 protein-protein interaction (PPI) by different
biochemical assays and report the high resolution crystal structure of a 13-mer
motif of SOS1 bound to 14-3-3ζ. These structural and functional insights are
important for the evaluation of this PPI interface for small-molecule
stabilization as a new starting point for modulating the Ras-Raf-MAPK pathway.
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Links
