PDBsum entry 6f08

Peptide binding protein

PDB id: 6f08

Contents

Protein chains

225 a.a.

Ligands

ARG-ARG-PRO-GLU-SEP-ALA-PRO-ALA-GLU

ARG-ARG-ARG-PRO-GLU-SEP-ALA-PRO-ALA-GLU

ARG-ARG-PRO-GLU-SEP-ALA-PRO-ALA

ARG-ARG-PRO-GLU-SEP-ALA-PRO-ALA-GLU-SER

1PE

Waters ×693

References listed in PDB file

Key reference

• Title: Structural characterization of 14-3-3ζ in complex with the human son of sevenless homolog 1 (sos1).
• Authors: A.Ballone, F.Centorrino, M.Wolter, C.Ottmann.
• Ref.: J Struct Biol, 2018, 202, 210-215. [DOI no: 10.1016/j.jsb.2018.01.011]
• PubMed id: 29408703

Abstract

The deviant Ras activation machinery is found in approximately 30% of all human cancers. SOS1 is an important protagonist of this pathway that plays a key-role in aberrant cell proliferation and differentiation. Interaction of SOS1 with 14-3-3 proteins modulates SOS1 activity in Ras-MAPK signaling. In the present study, we analyze the 14-3-3/SOS1 protein-protein interaction (PPI) by different biochemical assays and report the high resolution crystal structure of a 13-mer motif of SOS1 bound to 14-3-3ζ. These structural and functional insights are important for the evaluation of this PPI interface for small-molecule stabilization as a new starting point for modulating the Ras-Raf-MAPK pathway.