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PDBsum entry 5w3l
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Virus/immune system
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PDB id
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5w3l
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Contents |
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274 a.a.
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236 a.a.
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255 a.a.
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40 a.a.
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116 a.a.
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107 a.a.
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References listed in PDB file
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Key reference
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Title
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Antibody-Induced uncoating of human rhinovirus b14.
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Authors
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Y.Dong,
Y.Liu,
W.Jiang,
T.J.Smith,
Z.Xu,
M.G.Rossmann.
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Ref.
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Proc Natl Acad Sci U S A, 2017,
114,
8017-8022.
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PubMed id
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Abstract
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Rhinoviruses (RVs) are the major causes of common colds in humans. They have a
nonenveloped, icosahedral capsid surrounding a positive-strand RNA genome. Here
we report that the antigen-binding (Fab) fragment of a neutralizing antibody
(C5) can trigger genome release from RV-B14 to form emptied particles and
neutralize virus infection. Using cryo-electron microscopy, structures of the C5
Fab in complex with the full and emptied particles have been determined at 2.3
Å and 3.0 Å resolution, respectively. Each of the 60 Fab molecules binds
primarily to a region on viral protein 3 (VP3). Binding of the C5 Fabs to RV-B14
results in significant conformational changes around holes in the capsid through
which the viral RNA might exit. These results are so far the highest resolution
view of an antibody-virus complex and elucidate a mechanism whereby antibodies
neutralize RVs and related viruses by inducing virus uncoating.
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