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PDBsum entry 5oaf
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Gene regulation
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PDB id
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5oaf
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References listed in PDB file
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Key reference
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Title
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Cryo-Em structures of the human ino80 chromatin-Remodeling complex.
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Authors
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R.J.Aramayo,
O.Willhoft,
R.Ayala,
R.Bythell-Douglas,
D.B.Wigley,
X.Zhang.
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Ref.
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Nat Struct Mol Biol, 2018,
25,
37-44.
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PubMed id
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Abstract
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Access to chromatin for processes such as transcription and DNA repair requires
the sliding of nucleosomes along DNA. This process is aided by
chromatin-remodeling complexes, such as the multisubunit INO80
chromatin-remodeling complex. Here we present cryo-EM structures of the active
core complex of human INO80 at 9.6 Å, with portions at 4.1-Å resolution, and
reconstructions of combinations of subunits. Together, these structures reveal
the architecture of the INO80 complex, including Ino80 and actin-related
proteins, which is assembled around a single RUVBL1 (Tip49a) and RUVBL2 (Tip49b)
AAA+ heterohexamer. An unusual spoked-wheel structural domain of the Ino80
subunit is engulfed by this heterohexamer; both, in combination, form the core
of the complex. We also identify a cleft in RUVBL1 and RUVBL2, which forms a
major interaction site for partner proteins and probably communicates these
interactions to its nucleotide-binding sites.
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