PDBsum entry 5mqh

Transferase

PDB id: 5mqh

Contents

Protein chain

228 a.a.

Waters ×33

PDB id:

5mqh

Name:

Transferase

Title:

Structure of the phosphatase domain of the cell fate determinant spoiie from bacillus subtilis in a crystal form without domain swapping

Structure:

Serine phosphatase. Chain: a. Engineered: yes. Mutation: yes. Other_details: the mutation ala624 to ile was introduced to reduce successfully the extent of domain swapping.

Source:

Bacillus subtilis. Organism_taxid: 1423. Gene: sc09_contig28orf00413. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.45Å R-factor: 0.214 R-free: 0.278

Authors:

V.M.Levdikov,A.J.Wilkinson,E.V.Blagova

Key ref:

N.Bradshaw et al. (2017). A widespread family of serine/threonine protein phosp shares a common regulatory switch with proteasomal proteases.. Elife, 6, . PubMed id: 28527238

Date:

20-Dec-16 Release date: 31-May-17

Protein chain

P37475  (SP2E_BACSU) -  Stage II sporulation protein E from Bacillus subtilis (strain 168)

Seq: 827 a.a.

Struc: 228 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site