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PDBsum entry 5mg0
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References listed in PDB file
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Key reference
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Title
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Drop-On-Demand sample delivery for studying biocatalysts in action at X-Ray free-Electron lasers.
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Authors
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F.D.Fuller,
S.Gul,
R.Chatterjee,
E.S.Burgie,
I.D.Young,
H.Lebrette,
V.Srinivas,
A.S.Brewster,
T.Michels-Clark,
J.A.Clinger,
B.Andi,
M.Ibrahim,
E.Pastor,
C.De lichtenberg,
R.Hussein,
C.J.Pollock,
M.Zhang,
C.A.Stan,
T.Kroll,
T.Fransson,
C.Weninger,
M.Kubin,
P.Aller,
L.Lassalle,
P.Bräuer,
M.D.Miller,
M.Amin,
S.Koroidov,
C.G.Roessler,
M.Allaire,
R.G.Sierra,
P.T.Docker,
J.M.Glownia,
S.Nelson,
J.E.Koglin,
D.Zhu,
M.Chollet,
S.Song,
H.Lemke,
M.Liang,
D.Sokaras,
R.Alonso-Mori,
A.Zouni,
J.Messinger,
U.Bergmann,
A.K.Boal,
J.M.Bollinger,
C.Krebs,
M.Högbom,
G.N.Phillips,
R.D.Vierstra,
N.K.Sauter,
A.M.Orville,
J.Kern,
V.K.Yachandra,
J.Yano.
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Ref.
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Nat Methods, 2017,
14,
443-449.
[DOI no: ]
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PubMed id
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Abstract
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X-ray crystallography at X-ray free-electron laser sources is a powerful method
for studying macromolecules at biologically relevant temperatures. Moreover,
when combined with complementary techniques like X-ray emission spectroscopy,
both global structures and chemical properties of metalloenzymes can be obtained
concurrently, providing insights into the interplay between the protein
structure and dynamics and the chemistry at an active site. The implementation
of such a multimodal approach can be compromised by conflicting requirements to
optimize each individual method. In particular, the method used for sample
delivery greatly affects the data quality. We present here a robust way of
delivering controlled sample amounts on demand using acoustic droplet ejection
coupled with a conveyor belt drive that is optimized for crystallography and
spectroscopy measurements of photochemical and chemical reactions over a wide
range of time scales. Studies with photosystem II, the phytochrome
photoreceptor, and ribonucleotide reductase R2 illustrate the power and
versatility of this method.
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