PDBsum entry 5lab

Hydrolase

PDB id: 5lab

Contents

Protein chain

159 a.a.

Ligands

NGH

Metals

_ZN ×2

_CA ×3

Waters ×236

PDB id:

5lab

Name:

Hydrolase

Title:

Crystal structure of the catalytic domain of human mmp12 complexed with the inhibitor nngh

Structure:

Macrophage metalloelastase. Chain: a. Fragment: unp residues 106-263. Synonym: mme,macrophage elastase,hme,matrix metalloproteinase-12,mmp- 12. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mmp12, hme. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

1.34Å R-factor: 0.158 R-free: 0.195

Authors:

E.Ravera,V.Calderone,C.Luchinat

Key ref:

L.Benda et al. First-Principles calculation of pseudo-Contact shifts paramagnetic metalloprotein. To be published, . PubMed id: 27781358

Date:

14-Jun-16 Release date: 10-Aug-16

Protein chain

P39900  (MMP12_HUMAN) -  Macrophage metalloelastase from Homo sapiens

Seq: 470 a.a.

Struc: 159 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.24.65 - macrophage elastase.
• Reaction: Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in the B chain of insulin.
• Cofactor: Ca(2+); Zn(2+)