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PDBsum entry 5i2d
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Transcription/DNA/RNA
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PDB id
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5i2d
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Contents |
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284 a.a.
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1111 a.a.
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1363 a.a.
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94 a.a.
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346 a.a.
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195 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of transcription activation.
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Authors
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Y.Feng,
Y.Zhang,
R.H.Ebright.
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Ref.
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Science, 2016,
352,
1330-1333.
[DOI no: ]
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PubMed id
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Abstract
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Class II transcription activators function by binding to a DNA site overlapping
a core promoter and stimulating isomerization of an initial RNA polymerase
(RNAP)-promoter closed complex into a catalytically competent RNAP-promoter open
complex. Here, we report a 4.4 angstrom crystal structure of an intact bacterial
class II transcription activation complex. The structure comprises Thermus
thermophilus transcription activator protein TTHB099 (TAP) [homolog of
Escherichia coli catabolite activator protein (CAP)], T. thermophilus RNAP σ(A)
holoenzyme, a class II TAP-dependent promoter, and a ribotetranucleotide primer.
The structure reveals the interactions between RNAP holoenzyme and DNA
responsible for transcription initiation and reveals the interactions between
TAP and RNAP holoenzyme responsible for transcription activation. The structure
indicates that TAP stimulates isomerization through simple, adhesive,
stabilizing protein-protein interactions with RNAP holoenzyme.
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