PDBsum entry 5c8c

Virus

PDB id: 5c8c

Contents

Protein chains

259 a.a.

286 a.a.

242 a.a.

Ligands

STE

Metals

__K

_CL ×2

Waters ×506

PDB id:

5c8c

Name:

Virus

Title:

Crystal structure of recombinant coxsackievirus a16 capsid

Structure:

Vp1. Chain: a. Engineered: yes. Vp0. Chain: b. Engineered: yes. Vp3. Chain: c. Engineered: yes

Source:

Coxsackievirus a16 (strain tainan/5079/98). Organism_taxid: 231417. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.

Resolution:

2.50Å R-factor: 0.164 R-free: 0.172

Authors:

J.Ren,X.Wang,L.Zhu,Z.Hu,Q.Gao,P.Yang,X.Li,J.Wang,X.Shen,E.E.Fry, Z.Rao,D.I.Stuart

Key ref:

J.Ren et al. (2015). Structures of Coxsackievirus A16 Capsids with Native Antigenicity: Implications for Particle Expansion, Receptor Binding, and Immunogenicity. J Virol, 89, 10500-10511. PubMed id: 26269176 DOI: 10.1128/JVI.01102-15

Date:

25-Jun-15 Release date: 30-Sep-15

Protein chain

Q9QF31  (POLG_CX16T) -  Genome polyprotein from Coxsackievirus A16 (strain Tainan/5079/98)

Seq: 2193 a.a.

Struc: 259 a.a.*

Protein chain

Q9QF31  (POLG_CX16T) -  Genome polyprotein from Coxsackievirus A16 (strain Tainan/5079/98)

Seq: 2193 a.a.

Struc: 286 a.a.*

Protein chain

Q9QF31  (POLG_CX16T) -  Genome polyprotein from Coxsackievirus A16 (strain Tainan/5079/98)

Seq: 2193 a.a.

Struc: 242 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, B, C: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: Chains A, B, C: E.C.3.4.22.28 - picornain 3C.
• Reaction: Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 4: Chains A, B, C: E.C.3.4.22.29 - picornain 2A.
• Reaction: Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 5: Chains A, B, C: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1128/JVI.01102-15

J Virol 89:10500-10511 (2015)

PubMed id: 26269176

Structures of Coxsackievirus A16 Capsids with Native Antigenicity: Implications for Particle Expansion, Receptor Binding, and Immunogenicity.

J.Ren, X.Wang, L.Zhu, Z.Hu, Q.Gao, P.Yang, X.Li, J.Wang, X.Shen, E.E.Fry, Z.Rao, D.I.Stuart.

ABSTRACT

No abstract given.