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PDBsum entry 5byh
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Contents |
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305 a.a.
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216 a.a.
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1319 a.a.
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1372 a.a.
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79 a.a.
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421 a.a.
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References listed in PDB file
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Key reference
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Title
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Transcription. Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies.
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Authors
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Y.Yang,
V.C.Darbari,
N.Zhang,
D.Lu,
R.Glyde,
Y.P.Wang,
J.T.Winkelman,
R.L.Gourse,
K.S.Murakami,
M.Buck,
X.Zhang.
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Ref.
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Science, 2015,
349,
882-885.
[DOI no: ]
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PubMed id
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Abstract
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Transcription by RNA polymerase (RNAP) in bacteria requires specific promoter
recognition by σ factors. The major variant σ factor (σ(54)) initially forms
a transcriptionally silent complex requiring specialized adenosine
triphosphate-dependent activators for initiation. Our crystal structure of the
450-kilodalton RNAP-σ(54) holoenzyme at 3.8 angstroms reveals molecular details
of σ(54) and its interactions with RNAP. The structure explains how σ(54)
targets different regions in RNAP to exert its inhibitory function. Although
σ(54) and the major σ factor, σ(70), have similar functional domains and
contact similar regions of RNAP, unanticipated differences are observed in their
domain arrangement and interactions with RNAP, explaining their distinct
properties. Furthermore, we observe evolutionarily conserved regulatory hotspots
in RNAPs that can be targeted by a diverse range of mechanisms to fine tune
transcription.
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