PDBsum entry 4zr5

Hydrolase

PDB id: 4zr5

Contents

Protein chains

696 a.a.

Ligands

NAG-NAG ×4

RDF ×2

NAG ×2

Metals

_ZN ×2

Waters ×271

References listed in PDB file

Key reference

• Title: Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan.
• Authors: S.L.Labiuk, J.Sygusch, P.Grochulski.
• Ref.: Acta Crystallogr F Struct Biol Commun, 2019, 75, 405-411. [DOI no: 10.1107/S2053230X19006046]
• PubMed id: 31204686

Abstract

Neutral endopeptidase (neprilysin; NEP) is a proteinase that cleaves a wide variety of peptides and has been implicated in Alzheimer's disease, cardiovascular conditions, arthritis and other inflammatory diseases. The structure of the soluble extracellular domain (residues 55-750) of rabbit neprilysin was solved both in its native form at 2.1 Å resolution, and bound to the inhibitors phosphoramidon and thiorphan at 2.8 and 3.0 Å resolution, respectively. Consistent with the extracellular domain of human neprilysin, the structure reveals a large central cavity which contains the active site and the location for inhibitor binding.