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PDBsum entry 4zpy
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References listed in PDB file
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Key reference
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Title
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Structural basis for biologically relevant mechanical stiffening of a virus capsid by cavity-Creating or spacefilling mutations.
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Authors
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P.Guerra,
A.Valbuena,
J.Querol-Audí,
C.Silva,
M.Castellanos,
A.Rodríguez-Huete,
D.Garriga,
M.G.Mateu,
N.Verdaguer.
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Ref.
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Sci Rep, 2017,
7,
4101.
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PubMed id
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Abstract
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Recent studies reveal that the mechanical properties of virus particles may have
been shaped by evolution to facilitate virus survival. Manipulation of the
mechanical behavior of virus capsids is leading to a better understanding of
viral infection, and to the development of virus-based nanoparticles with
improved mechanical properties for nanotechnological applications. In the minute
virus of mice (MVM), deleterious mutations around capsid pores involved in
infection-related translocation events invariably increased local mechanical
stiffness and interfered with pore-associated dynamics. To provide
atomic-resolution insights into biologically relevant changes in virus capsid
mechanics, we have determined by X-ray crystallography the structural effects of
deleterious, mechanically stiffening mutations around the capsid pores. Data
show that the cavity-creating N170A mutation at the pore wall does not induce
any dramatic structural change around the pores, but instead generates subtle
rearrangements that propagate throughout the capsid, resulting in a more
compact, less flexible structure. Analysis of the spacefilling L172W mutation
revealed the same relationship between increased stiffness and compacted capsid
structure. Implications for understanding connections between virus mechanics,
structure, dynamics and infectivity, and for engineering modified virus-based
nanoparticles, are discussed.
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