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PDBsum entry 4zk7
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Protein binding
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PDB id
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4zk7
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Contents |
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(+ 6 more)
117 a.a.
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(+ 5 more)
103 a.a.
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109 a.a.
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References listed in PDB file
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Key reference
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Title
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Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.
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Authors
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J.B.Bale,
R.U.Park,
Y.Liu,
S.Gonen,
T.Gonen,
D.Cascio,
N.P.King,
T.O.Yeates,
D.Baker.
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Ref.
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Protein Sci, 2015,
24,
1695-1701.
[DOI no: ]
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PubMed id
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Abstract
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We recently reported the development of a computational method for the design of
coassembling multicomponent protein nanomaterials. While four such materials
were validated at high-resolution by X-ray crystallography, low yield of soluble
protein prevented X-ray structure determination of a fifth designed material,
T33-09. Here we report the design and crystal structure of T33-31, a variant of
T33-09 with improved soluble yield resulting from redesign efforts focused on
mutating solvent-exposed side chains to charged amino acids. The structure is
found to match the computational design model with atomic-level accuracy,
providing further validation of the design approach and demonstrating a simple
and potentially general means of improving the yield of designed protein
nanomaterials.
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