PDBsum entry 4z7c

Lyase

PDB id: 4z7c

Contents

Protein chain

324 a.a.

Ligands

PO4 ×2

GOL

Metals

_NA

Waters ×140

PDB id:

4z7c

Name:

Lyase

Title:

Diphosphomevalonate decarboxylase from the sulfolobus solfataricus, space group h32

Structure:

Diphosphomevalonate decarboxylase. Chain: a. Fragment: unp residues 2-325. Synonym: dmd. Engineered: yes

Source:

Sulfolobus solfataricus (strain atcc 35092 / dsm 1617 / jcm 11322 / p2). Organism_taxid: 273057. Strain: atcc 35092 / dsm 1617 / jcm 11322 / p2. Gene: sso2989. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.20Å R-factor: 0.212 R-free: 0.237

Authors:

H.Unno,A.Hattori,H.Hemmi

Key ref:

A.Hattori et al. (2015). In Vivo Formation of the Protein Disulfide Bond That Enhances the Thermostability of Diphosphomevalonate Decarboxylase, an Intracellular Enzyme from the Hyperthermophilic Archaeon Sulfolobus solfataricus. J Bacteriol, 197, 3463-3471. PubMed id: 26303832 DOI: 10.1128/JB.00352-15

Date:

07-Apr-15 Release date: 16-Sep-15

Protein chain

Q97UL5  (DMD_SULSO) -  Diphosphomevalonate decarboxylase from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Seq: 325 a.a.

Struc: 324 a.a.

Enzyme reactions

• Enzyme class: E.C.4.1.1.33 - diphosphomevalonate decarboxylase.
• Pathway: Terpenoid biosynthesis
• Reaction: (R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2

(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2 (Bound ligand (Het Group name = PO4) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1128/JB.00352-15

J Bacteriol 197:3463-3471 (2015)

PubMed id: 26303832

In Vivo Formation of the Protein Disulfide Bond That Enhances the Thermostability of Diphosphomevalonate Decarboxylase, an Intracellular Enzyme from the Hyperthermophilic Archaeon Sulfolobus solfataricus.

A.Hattori, H.Unno, S.Goda, K.Motoyama, T.Yoshimura, H.Hemmi.

ABSTRACT

No abstract given.