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PDBsum entry 4z0c
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Immune system
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PDB id
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4z0c
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References listed in PDB file
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Key reference
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Title
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Structural basis for specific recognition of single-Stranded RNA by toll-Like receptor 13.
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Authors
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W.Song,
J.Wang,
Z.Han,
Y.Zhang,
H.Zhang,
W.Wang,
J.Chang,
B.Xia,
S.Fan,
D.Zhang,
J.Wang,
H.W.Wang,
J.Chai.
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Ref.
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Nat Struct Biol, 2015,
22,
782-787.
[DOI no: ]
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PubMed id
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Abstract
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Toll-like receptors (TLRs) have crucial roles in innate immunity, functioning as
pattern-recognition receptors. TLR13 recognizes a conserved sequence from
bacterial 23S rRNA and then triggers an immune response. Here we report the
crystal structure of the mouse TLR13 ectodomain bound by a 13-nt single-stranded
(ss) RNA derived from 23S rRNA. The ssRNA induces TLR13 dimerization but assumes
a stem-loop-like structure that is completely different from that in the
bacterial ribosome but nevertheless is crucial for TLR13 recognition. Most of
the RNA nucleotides are splayed out to make base-specific contacts with the
concave surface of TLR13, and RNA-specific interactions are important to allow
TLR13 to distinguish RNA from DNA. Interestingly, a viral-derived 16-nt ssRNA
predicted to form a similar stem-loop-like structure also induces TLR13
activation. Together, our results reveal the structural mechanism of TLR13's
sequence- and conformation-specific recognition of ssRNA.
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