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PDBsum entry 4xjc
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References listed in PDB file
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Key reference
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Title
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Bacillus halodurans strain c125 encodes and synthesizes enzymes from both known pathways to form dump directly from cytosine deoxyribonucleotides.
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Authors
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C.B.Oehlenschlæger,
M.N.Løvgreen,
E.Reinauer,
E.Lehtinen,
M.L.Pind,
P.Harris,
J.Martinussen,
M.Willemoës.
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Ref.
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Appl Environ Microbiol, 2015,
81,
3395-3404.
[DOI no: ]
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PubMed id
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Abstract
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Analysis of the genome of Bacillus halodurans strain C125 indicated that two
pathways leading from a cytosine deoxyribonucleotide to dUMP, used for dTMP
synthesis, were encoded by the genome of the bacterium. The genes that were
responsible, the comEB gene and the dcdB gene, encoding dCMP deaminase and the
bifunctional dCTP deaminase:dUTPase (DCD:DUT), respectively, were both shown to
be expressed in B. halodurans, and both genes were subject to repression by the
nucleosides thymidine and deoxycytidine. The latter nucleoside presumably exerts
its repression after deamination by cytidine deaminase. Both comEB and dcdB were
cloned, overexpressed in Escherichia coli, and purified to homogeneity. Both
enzymes were active and displayed the expected regulatory properties: activation
by dCTP for dCMP deaminase and dTTP inhibition for both enzymes. Structurally,
the B. halodurans enzyme resembled the Mycobacterium tuberculosis enzyme the
most. An investigation of sequenced genomes from other species of the genus
Bacillus revealed that not only the genome of B. halodurans but also the genomes
of Bacillus pseudofirmus, Bacillus thuringiensis, Bacillus hemicellulosilyticus,
Bacillus marmarensis, Bacillus cereus, and Bacillus megaterium encode both the
dCMP deaminase and the DCD:DUT enzymes. In addition, eight dcdB homologs from
Bacillus species within the genus for which the whole genome has not yet been
sequenced were registered in the NCBI Entrez database.
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