 |
PDBsum entry 4fc5
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Unknown function
|
PDB id
|
|
|
|
4fc5
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Unknown function
|
|
|
Title:
|
|
Crystal structure of ton_0340
|
|
Structure:
|
|
Putative uncharacterized protein. Chain: a, b, c, d, e, f. Synonym: ton_0340. Engineered: yes
|
|
Source:
|
|
Thermococcus onnurineus. Organism_taxid: 523850. Strain: na1. Gene: ton_0340. Expressed in: escherichia coli. Expression_system_taxid: 562.
|
|
Resolution:
|
|
|
2.30Å
|
R-factor:
|
0.223
|
R-free:
|
0.281
|
|
|
Authors:
|
|
S.G.Lee,K.H.Lee,Y.J.An,S.S.Cha,B.H.Oh
|
|
Key ref:
|
|
S.S.Cha
et al.
(2012).
Experimental phasing using zinc anomalous scattering.
Acta Crystallogr D Biol Crystallogr,
68,
1253-1258.
PubMed id:
|
|
|
Date:
|
|
|
24-May-12
|
Release date:
|
12-Sep-12
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
B6YTD8
(B6YTD8_THEON) -
D-glutamate cyclase-like C-terminal domain-containing protein from Thermococcus onnurineus (strain NA1)
|
|
|
|
Seq:
Struc:
|
|
|
|
268 a.a.
259 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
Acta Crystallogr D Biol Crystallogr
68:1253-1258
(2012)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Experimental phasing using zinc anomalous scattering.
|
|
S.S.Cha,
Y.J.An,
C.S.Jeong,
M.K.Kim,
S.G.Lee,
K.H.Lee,
B.H.Oh.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
Zinc is a suitable metal for anomalous dispersion phasing methods in protein
crystallography. Structure determination using zinc anomalous scattering has
been almost exclusively limited to proteins with intrinsically bound zinc(s).
Here, it is reported that multiple zinc ions can easily be charged onto the
surface of proteins with no intrinsic zinc-binding site by using zinc-containing
solutions. Zn derivatization of protein surfaces appears to be a largely
unnoticed but promising method of protein structure determination.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
