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PDBsum entry 4fc5
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Unknown function
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PDB id
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4fc5
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References listed in PDB file
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Key reference
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Title
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Experimental phasing using zinc anomalous scattering.
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Authors
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S.S.Cha,
Y.J.An,
C.S.Jeong,
M.K.Kim,
S.G.Lee,
K.H.Lee,
B.H.Oh.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2012,
68,
1253-1258.
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PubMed id
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Abstract
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Zinc is a suitable metal for anomalous dispersion phasing methods in protein
crystallography. Structure determination using zinc anomalous scattering has
been almost exclusively limited to proteins with intrinsically bound zinc(s).
Here, it is reported that multiple zinc ions can easily be charged onto the
surface of proteins with no intrinsic zinc-binding site by using zinc-containing
solutions. Zn derivatization of protein surfaces appears to be a largely
unnoticed but promising method of protein structure determination.
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