PDBsum entry 4dxb

Sugar binding protein, hydrolase

PDB id: 4dxb

Contents

Protein chains

637 a.a.

Metals

_ZN ×4

Waters ×490

PDB id:

4dxb

Name:

Sugar binding protein, hydrolase

Title:

2.29a structure of the engineered mbp tem-1 fusion protein rg13 in complex with zinc, p1 space group

Structure:

Maltose-binding periplasmic protein, beta-lactamase tem chimera. Chain: a, b. Fragment: see remark 999. Synonym: rg13. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: male, bla. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.29Å R-factor: 0.235 R-free: 0.292

Authors:

F.Van Den Akker,W.Ke

Key ref:

W.Ke et al. (2012). Structure of an engineered β-lactamase maltose binding protein fusion protein: insights into heterotropic allosteric regulation. Plos One, 7, e39168. PubMed id: 22720063

Date:

27-Feb-12 Release date: 08-Aug-12

Protein chains

P0AEX9  (MALE_ECOLI) -  Maltose/maltodextrin-binding periplasmic protein from Escherichia coli (strain K12)

Seq: 396 a.a.

Struc: 637 a.a.*

Protein chains

P62593  (BLAT_ECOLX) -  Beta-lactamase TEM from Escherichia coli

Seq: 286 a.a.

Struc: 637 a.a.*

* PDB and UniProt seqs differ at 114 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.5.2.6 - beta-lactamase.
• Pathway: Penicillin Biosynthesis and Metabolism
• Reaction: a beta-lactam + H2O = a substituted beta-amino acid
• Cofactor: Zn(2+)

Plos One 7:e39168 (2012)

PubMed id: 22720063

Structure of an engineered β-lactamase maltose binding protein fusion protein: insights into heterotropic allosteric regulation.

W.Ke, A.H.Laurent, M.D.Armstrong, Y.Chen, W.E.Smith, J.Liang, C.M.Wright, M.Ostermeier, F.van den Akker.

ABSTRACT

No abstract given.