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PDBsum entry 4d2e
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protein ligands metals Protein-protein interface(s) links
Transferase PDB id
4d2e

 

 

 

 

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JSmol PyMol  
Contents
Protein chains
116 a.a.
98 a.a.
93 a.a.
108 a.a.
90 a.a.
Ligands
78M ×7
78N ×11
FLC ×2
Metals
_NA
_ZN
Waters ×144
PDB id:
4d2e
Name: Transferase
Title: Crystal structure of an integral membrane kinase - v2.3
Structure: Diacylglycerol kinase. Chain: a, b, c, d, e, f. Synonym: dagk, diglyceride kinase, dgk. Engineered: yes. Mutation: yes
Source: Escherichia coli k-12. Organism_taxid: 83333. Atcc: 29425. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: cell-free expression
Resolution:
2.28Å     R-factor:   0.190     R-free:   0.229
Authors: D.Li,C.Boland,M.Caffrey
Key ref: C.Boland et al. (2014). Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination. Cell Mol Life Sci, 71, 4895-4910. PubMed id: 25012698 DOI: 10.1007/s00018-014-1655-7
Date:
09-May-14     Release date:   23-Jul-14    
PROCHECK
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 Headers
 References

Protein chain
P0ABN1  (KDGL_ECOLI) -  Diacylglycerol kinase from Escherichia coli (strain K12)
Seq:
Struc: 		122 a.a.
116 a.a.*
Protein chain
P0ABN1  (KDGL_ECOLI) -  Diacylglycerol kinase from Escherichia coli (strain K12)
Seq:
Struc: 		122 a.a.
98 a.a.*
Protein chains
P0ABN1  (KDGL_ECOLI) -  Diacylglycerol kinase from Escherichia coli (strain K12)
Seq:
Struc: 		122 a.a.
93 a.a.*
Protein chain
P0ABN1  (KDGL_ECOLI) -  Diacylglycerol kinase from Escherichia coli (strain K12)
Seq:
Struc: 		122 a.a.
108 a.a.*
Protein chain
P0ABN1  (KDGL_ECOLI) -  Diacylglycerol kinase from Escherichia coli (strain K12)
Seq:
Struc: 		122 a.a.
90 a.a.*
Key:    Secondary structure  CATH domain
* PDB and UniProt seqs differ at 35 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D, E, F: E.C.2.7.1.107  - diacylglycerol kinase (ATP).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H+
1,2-diacyl-sn-glycerol
 + ATP
 = 1,2-diacyl-sn-glycero-3-phosphate
 + ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
DOI no: 10.1007/s00018-014-1655-7 Cell Mol Life Sci 71:4895-4910 (2014)
PubMed id: 25012698  
 
 
Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination.
C.Boland, D.Li, S.T.Shah, S.Haberstock, V.Dötsch, F.Bernhard, M.Caffrey.
 
  ABSTRACT  
 
No abstract given.

 

 

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