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PDBsum entry 4ayb
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Contents |
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872 a.a.
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1103 a.a.
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376 a.a.
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262 a.a.
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171 a.a.
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105 a.a.
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113 a.a.
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76 a.a.
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84 a.a.
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91 a.a.
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65 a.a.
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44 a.a.
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50 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural and functional analyses of the interaction of archaeal RNA polymerase with DNA.
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Authors
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M.N.Wojtas,
M.Mogni,
O.Millet,
S.D.Bell,
N.G.Abrescia.
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Ref.
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Nucleic Acids Res, 2012,
40,
9941-9952.
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PubMed id
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Abstract
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Multi-subunit RNA polymerases (RNAPs) in all three domains of life share a
common ancestry. The composition of the archaeal RNAP (aRNAP) is not identical
between phyla and species, with subunits Rpo8 and Rpo13 found in restricted
subsets of archaea. While Rpo8 has an ortholog, Rpb8, in the nuclear eukaryal
RNAPs, Rpo13 lacks clear eukaryal orthologs. Here, we report crystal structures
of the DNA-bound and free form of the aRNAP from Sulfolobus shibatae. Together
with biochemical and biophysical analyses, these data show that Rpo13 C-terminus
binds non-specifically to double-stranded DNA. These interactions map on our
RNAP-DNA binary complex on the downstream DNA at the far end of the DNA entry
channel. Our findings thus support Rpo13 as a RNAP-DNA stabilization factor, a
role reminiscent of eukaryotic general transcriptional factors. The data further
yield insight into the mechanisms and evolution of RNAP-DNA interaction.
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Secondary reference #1
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Title
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Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the rpo13 subunit.
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Authors
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M.Wojtas,
B.Peralta,
M.Ondiviela,
M.Mogni,
S.D.Bell,
N.G.Abrescia.
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Ref.
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Biochem Soc Trans, 2011,
39,
25-30.
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PubMed id
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Secondary reference #2
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Title
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Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure.
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Authors
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Y.Korkhin,
U.M.Unligil,
O.Littlefield,
P.J.Nelson,
D.I.Stuart,
P.B.Sigler,
S.D.Bell,
N.G.Abrescia.
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Ref.
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Plos Biol, 2009,
7,
e1000102.
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PubMed id
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