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PDBsum entry 4aa6
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Transcription/DNA
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PDB id
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4aa6
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Contents |
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69 a.a.
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70 a.a.
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68 a.a.
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69 a.a.
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References listed in PDB file
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Key reference
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Title
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The oestrogen receptor recognizes an imperfectly palindromic response element through an alternative side-Chain conformation.
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Authors
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J.W.Schwabe,
L.Chapman,
D.Rhodes.
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Ref.
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Structure, 1995,
3,
201-213.
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PubMed id
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Abstract
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BACKGROUND: Structural studies of protein-DNA complexes have tended to give the
impression that DNA recognition requires a unique molecular interface. However,
many proteins recognize DNA targets that differ from what is thought to be their
ideal target sequence. The steroid hormone receptors illustrate this problem in
recognition rather well, since consensus DNA targets are rare. RESULTS: Here we
describe the structure, at 2.6 A resolution, of a complex between a dimer of the
DNA-binding domain from the human oestrogen receptor (ERDBD) and a non-consensus
DNA target site in which there is a single base substitution in one half of the
palindromic binding site. This substitution results in a 10-fold increase in the
dissociation constant of the ERDBD-DNA complex. Comparison of this structure
with a structure containing a consensus DNA-binding site determined previously,
shows that recognition of the non-consensus sequence is achieved by the
rearrangement of a lysine side chain so as to make an alternative base contact.
CONCLUSIONS: This study suggests that proteins adapt to recognize different DNA
sequences by rearranging side chains at the protein-DNA interface so as to form
alternative patterns of intermolecular contacts.
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