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PDBsum entry 3v9u
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Hydrolase/DNA
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PDB id
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3v9u
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PDB id:
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| Name: |
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Hydrolase/DNA
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Title:
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Crystal structure of rnase t in complex with a preferred ssdna (aat) with two mg in the active site
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Structure:
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Ribonuclease t. Chain: a, b, c, d. Synonym: exoribonuclease t, rnase t. Engineered: yes. Mutation: yes. DNA (5'-d( Tp Tp Ap Cp Ap Ap T)-3'). Chain: e, f, g, h. Engineered: yes
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Source:
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Escherichia coli. Organism_taxid: 83333. Strain: k-12. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic construct
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Resolution:
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2.30Å
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R-factor:
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0.190
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R-free:
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0.245
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Authors:
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Y.-Y.Hsiao,H.S.Yuan
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Key ref:
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Y.Y.Hsiao
et al.
(2012).
How an exonuclease decides where to stop in trimming of nucleic acids: crystal structures of RNase T-product complexes.
Nucleic Acids Res,
40,
8144-8154.
PubMed id:
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Date:
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28-Dec-11
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Release date:
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11-Jul-12
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PROCHECK
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Headers
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References
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P30014
(RNT_ECOLI) -
Ribonuclease T from Escherichia coli (strain K12)
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Seq:
Struc:
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215 a.a.
206 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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A-A-T
3 bases
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A-A-T
3 bases
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A-A-T
3 bases
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Nucleic Acids Res
40:8144-8154
(2012)
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PubMed id:
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How an exonuclease decides where to stop in trimming of nucleic acids: crystal structures of RNase T-product complexes.
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Y.Y.Hsiao,
Y.Duh,
Y.P.Chen,
Y.T.Wang,
H.S.Yuan.
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ABSTRACT
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Links
