PDBsum entry 3v1v

Lyase

PDB id: 3v1v

Contents

Protein chain

317 a.a.

Ligands

GST

GOL

Metals

_MG ×2

Waters ×426

PDB id:

3v1v

Name:

Lyase

Title:

Crystal structure of 2-methylisoborneol synthase from streptomyces coelicolor a3(2) in complex with mg2+ and geranyl-s-thiolodiphosphate

Structure:

2-methylisoborneol synthase. Chain: a. Fragment: unp residues 29-440. Synonym: 2-mib synthase. Engineered: yes

Source:

Streptomyces coelicolor. Organism_taxid: 100226. Strain: a3(2). Gene: sc1a4.08, scbac12c8.01, sco7700. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.152 R-free: 0.176

Authors:

M.Koksal,D.W.Christianson

Key ref:

M.Köksal et al. (2012). Structure of 2-methylisoborneol synthase from Streptomyces coelicolor and implications for the cyclization of a noncanonical C-methylated monoterpenoid substrate. Biochemistry, 51, 3011-3020. PubMed id: 22455514

Date:

10-Dec-11 Release date: 11-Apr-12

Protein chain

Q9F1Y6  (MIBS_STRCO) -  2-methylisoborneol synthase from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

Seq: 440 a.a.

Struc: 317 a.a.

Enzyme reactions

• Enzyme class: E.C.4.2.3.118 - 2-methylisoborneol synthase.
• Reaction: (E)-2-methylgeranyl diphosphate + H2O = 2-methylisoborneol + diphosphate

(E)-2-methylgeranyl diphosphate + H2O = 2-methylisoborneol + diphosphate (Bound ligand (Het Group name = GST) matches with 40.00% similarity)

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

Biochemistry 51:3011-3020 (2012)

PubMed id: 22455514

Structure of 2-methylisoborneol synthase from Streptomyces coelicolor and implications for the cyclization of a noncanonical C-methylated monoterpenoid substrate.

M.Köksal, W.K.Chou, D.E.Cane, D.W.Christianson.

ABSTRACT

No abstract given.