 |
PDBsum entry 3spd
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Hydrolase/DNA
|
PDB id
|
|
|
|
3spd
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Crystal structures of aprataxin ortholog hnt3 reveal the mechanism for reversal of 5'-Adenylated DNA.
|
|
|
Authors
|
|
Y.Gong,
D.Zhu,
J.Ding,
C.N.Dou,
X.Ren,
L.Gu,
T.Jiang,
D.C.Wang.
|
|
|
Ref.
|
|
Nat Struct Biol, 2011,
18,
1297-1299.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Aprataxin is a DNA deadenylase that resolves DNA 5'-AMP termini and reverses
abortive DNA ligation. The crystal structures of Schizosaccharomyces pombe
aprataxin Hnt3 in its apo form and in complex to dsDNA and dsDNA-AMP reveal how
Hnt3 recognizes and processes 5'-adenylated DNA in a structure-specific manner.
The bound DNA adopts a 5'-flap conformation that facilitates 5'-AMP access to
the active site, where AMP cleavage occurs by a canonical catalytic mechanism.
|
|
|
|
|
|
|
