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PDBsum entry 3ho7
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Transcription
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PDB id
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3ho7
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References listed in PDB file
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Key reference
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Title
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Structures of the porphyromonas gingivalis oxyr regulatory domain explain differences in expression of the oxyr regulon in escherichia coli and p. Gingivalis.
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Authors
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D.V.Svintradze,
D.L.Peterson,
E.A.Collazo-Santiago,
J.P.Lewis,
H.T.Wright.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2013,
69,
2091-2103.
[DOI no: ]
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PubMed id
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Abstract
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OxyR transcriptionally regulates Escherichia coli oxidative stress response
genes through a reversibly reducible cysteine disulfide biosensor of cellular
redox status. Structural changes induced by redox changes in these cysteines are
conformationally transmitted to the dimer subunit interfaces, which alters dimer
and tetramer interactions with DNA. In contrast to E. coli OxyR
regulatory-domain structures, crystal structures of Porphyromonas gingivalis
OxyR regulatory domains show minimal differences in dimer configuration on
changes in cysteine disulfide redox status. This locked configuration of the P.
gingivalis OxyR regulatory-domain dimer closely resembles the oxidized
(activating) form of the E. coli OxyR regulatory-domain dimer. It correlates
with the observed constitutive activation of some oxidative stress genes in
P. gingivalis and is attributable to a single amino-acid insertion in P.
gingivalis OxyR relative to E. coli OxyR. Modelling of full-length P.
gingivalis, E. coli and Neisseria meningitidis OxyR-DNA complexes predicts
different modes of DNA binding for the reduced and oxidized forms of each.
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