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PDBsum entry 3gfi
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Transcription/DNA
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PDB id
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3gfi
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References listed in PDB file
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Key reference
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Title
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St1710-Dna complex crystal structure reveals the DNA binding mechanism of the marr family of regulators.
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Authors
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T.Kumarevel,
T.Tanaka,
T.Umehara,
S.Yokoyama.
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Ref.
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Nucleic Acids Res, 2009,
37,
4723-4735.
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PubMed id
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Abstract
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ST1710, a member of the multiple antibiotic resistance regulator (MarR) family
of regulatory proteins in bacteria and archaea, plays important roles in
development of antibiotic resistance, a global health problem. Here, we present
the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with
salicylate, a well-known inhibitor of MarR proteins and the ST1710 complex with
its promoter DNA, refined to 1.8 and 2.10 A resolutions, respectively. The
ST1710-DNA complex shares the topology of apo-ST1710 and MarR proteins, with
each subunit containing a winged helix-turn-helix (wHtH) DNA binding motif.
Significantly large conformational changes occurred upon DNA binding and in each
of the dimeric monomers in the asymmetric unit of the ST1710-DNA complex.
Conserved wHtH loop residues interacting with the bound DNA and mutagenic
analysis indicated that R89, R90 and K91 were important for DNA recognition.
Significantly, the bound DNA exhibited a new binding mechanism.
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Secondary reference #1
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Title
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Crystal structure of the marr family regulatory protein, St1710, From sulfolobus tokodaii strain 7.
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Authors
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T.Kumarevel,
T.Tanaka,
M.Nishio,
S.C.Gopinath,
K.Takio,
A.Shinkai,
P.K.Kumar,
S.Yokoyama.
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Ref.
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J Struct Biol, 2008,
161,
9.
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PubMed id
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