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PDBsum entry 3fd2
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Hydrolase/DNA
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PDB id
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3fd2
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References listed in PDB file
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Key reference
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Title
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Generation of single-Chain laglidadg homing endonucleases from native homodimeric precursor proteins.
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Authors
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H.Li,
S.Pellenz,
U.Ulge,
B.L.Stoddard,
R.J.Monnat.
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Ref.
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Nucleic Acids Res, 2009,
37,
1650-1662.
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PubMed id
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Abstract
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Homing endonucleases (HEs) cut long DNA target sites with high specificity to
initiate and target the lateral transfer of mobile introns or inteins. This high
site specificity of HEs makes them attractive reagents for gene targeting to
promote DNA modification or repair. We have generated several hundred
catalytically active, monomerized versions of the well-characterized homodimeric
I-CreI and I-MsoI LAGLIDADG family homing endonuclease (LHE) proteins.
Representative monomerized I-CreI and I-MsoI proteins (collectively termed
mCreIs or mMsoIs) were characterized in detail by using a combination of
biochemical, biophysical and structural approaches. We also demonstrated that
both mCreI and mMsoI proteins can promote cleavage-dependent recombination in
human cells. The use of single chain LHEs should simplify gene modification and
targeting by requiring the expression of a single small protein in cells, rather
than the coordinate expression of two separate protein coding genes as is
required when using engineered heterodimeric zinc finger or homing endonuclease
proteins.
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