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PDBsum entry 2y1j
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Transferase/DNA
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PDB id
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2y1j
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References listed in PDB file
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Key reference
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Title
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Crystal structures and repair studies reveal the identity and the base-Pairing properties of the uv-Induced spore photoproduct DNA lesion.
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Authors
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K.Heil,
A.C.Kneuttinger,
S.Schneider,
U.Lischke,
T.Carell.
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Ref.
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Chemistry, 2011,
17,
9651-9657.
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PubMed id
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Abstract
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UV light is one of the major causes of DNA damage. In spore DNA, due to an
unusual packing of the genetic material, a special spore photoproduct lesion (SP
lesion) is formed, which is repaired by the enzyme spore photoproduct lyase
(Spl), a radical S-adenosylmethionine (SAM) enzyme. We report here the synthesis
and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester
backbone. The oligonucleotides were used for repair studies and they were
cocrystallized with a polymerase enzyme as a template to clarify the
configuration of the SP lesion and to provide information about the base-pairing
properties of the lesion. The structural analysis together with repair studies
allowed us to clarify the identity of the preferentially repaired lesion
diastereoisomer.
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