PDBsum entry 2xcp

DNA/transferase

PDB id

2xcp

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Contents

			Protein chains

					342 a.a. *

			DNA/RNA

			Ligands

					DCP ×2

			Metals

					_MG ×6

			Waters ×305

* Residue conservation analysis

PDB id:

2xcp

Links

Name:

DNA/transferase

Title:

Ternary complex of sulfolobus solfataricus dpo4 DNA polymerase, 7,8- dihydro-8-oxodeoxyguanine modified DNA and dctp - magnesium form

Structure:

DNA polymerase iv. Chain: a, b. Synonym: pol iv. Engineered: yes. 5'-d( Gp Gp Gp Gp Gp Ap Ap Gp Gp Ap Tp Tp Doc)-3'. Chain: e, p. Engineered: yes. Other_details: 13-mer primer. 5'-d( Tp Cp Ap Cp 8Ogp Gp Ap Ap Tp Cp Cp

Source:

Sulfolobus solfataricus. Organism_taxid: 273057. Strain: p2. Gene: dpo4. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic: yes

Resolution:

2.60Å

R-factor:

0.223

R-free:

0.268

Authors:

A.Irimia,M.Egli

Key ref:

A.Irimia et al. (2010). Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus. Acta Crystallogr Sect F Struct Biol Cryst Commun, 66, 1013-1018. PubMed id: 20823515

Date:

24-Apr-10

Release date:

08-Sep-10

PROCHECK

	Headers

	References

Protein chains

Q97W02 (DPO4_SULSO) - DNA polymerase IV from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Seq: Struc:					352 a.a. 342 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

DNA/RNA chains

		G-G-G-G-G-A-A-G-G-A-T-T-DOC 13 bases
		T-C-A-C-8OG-G-A-A-T-C-C-T-T-C-C-C-C-C 18 bases
		G-G-G-G-G-A-A-G-G-A-T-T-DOC 13 bases
		T-C-A-C-8OG-G-A-A-T-C-C-T-T-C-C-C-C-C 18 bases



		Enzyme reactions

Enzyme class:

E.C.2.7.7.7 - DNA-directed Dna polymerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

DNA(n)	+	2'-deoxyribonucleoside 5'-triphosphate	=	DNA(n+1)	+	diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Acta Crystallogr Sect F Struct Biol Cryst Commun 66:1013-1018 (2010)
PubMed id: 20823515

Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus.
A.Irimia, L.V.Loukachevitch, R.L.Eoff, F.P.Guengerich, M.Egli.

ABSTRACT

No abstract given.