PDBsum entry 2x1f

Transcription/RNA

PDB id

2x1f

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Contents

			Protein chain

					94 a.a. *

			Ligands

					__G-__U

			Waters ×122

* Residue conservation analysis

PDB id:

2x1f

Links

Name:

Transcription/RNA

Title:

Structure of rna15 rrm with bound RNA (gu)

Structure:

mRNA 3'-end-processing protein rna15. Chain: a. Fragment: RNA recognition module, residues 16-103. Engineered: yes. 5'-r( Gp Up Up Gp Up)-3'. Chain: b. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

1.60Å

R-factor:

0.210

R-free:

0.240

Authors:

C.Pancevac,D.C.Goldstone,A.Ramos,I.A.Taylor

Key ref:

C.Pancevac et al. (2010). Structure of the Rna15 RRM-RNA complex reveals the molecular basis of GU specificity in transcriptional 3'-end processing factors. Nucleic Acids Res, 38, 3119-3132. PubMed id: 20097654

Date:

06-Jan-10

Release date:

02-Feb-10

PROCHECK

	Headers

	References

Protein chain

P25299 (RNA15_YEAST) - mRNA 3'-end-processing protein RNA15 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: Struc:					296 a.a. 94 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

	Nucleic Acids Res 38:3119-3132 (2010)
PubMed id: 20097654

Structure of the Rna15 RRM-RNA complex reveals the molecular basis of GU specificity in transcriptional 3'-end processing factors.
C.Pancevac, D.C.Goldstone, A.Ramos, I.A.Taylor.

ABSTRACT

Rna15 is a core subunit of cleavage factor IA (CFIA), an essential transcriptional 3'-end processing factor from Saccharomyces cerevisiae. CFIA is required for polyA site selection/cleavage targeting RNA sequences that surround polyadenylation sites in the 3'-UTR of RNA polymerase-II transcripts. RNA recognition by CFIA is mediated by an RNA recognition motif (RRM) contained in the Rna15 subunit of the complex. We show here that Rna15 has a strong and unexpected preference for GU containing RNAs and reveal the molecular basis for a base selectivity mechanism that accommodates G or U but discriminates against C and A bases. This mode of base selectivity is rather different to that observed in other RRM-RNA structures and is structurally conserved in CstF64, the mammalian counterpart of Rna15. Our observations provide evidence for a highly conserved mechanism of base recognition amongst the 3'-end processing complexes that interact with the U-rich or U/G-rich elements at 3'-end cleavage/polyadenylation sites.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21481776

M.Moreno-Morcillo, L.Minvielle-Sébastia, S.Fribourg, and C.D.Mackereth (2011).
Locked tether formation by cooperative folding of Rna14p monkeytail and Rna15p hinge domains in the yeast CF IA complex.

Structure, 19, 534-545.

PDB code:

2l9b

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.