PDBsum entry 2x1f

Transcription/RNA

PDB id: 2x1f

Contents

Protein chain

94 a.a.

Ligands

__G-__U

Waters ×122

References listed in PDB file

Key reference

• Title: Structure of the RNA15 rrm-Rna complex reveals the molecular basis of gu specificity in transcriptional 3'-End processing factors.
• Authors: C.Pancevac, D.C.Goldstone, A.Ramos, I.A.Taylor.
• Ref.: Nucleic Acids Res, 2010, 38, 3119-3132.
• PubMed id: 20097654

Abstract

Rna15 is a core subunit of cleavage factor IA (CFIA), an essential transcriptional 3'-end processing factor from Saccharomyces cerevisiae. CFIA is required for polyA site selection/cleavage targeting RNA sequences that surround polyadenylation sites in the 3'-UTR of RNA polymerase-II transcripts. RNA recognition by CFIA is mediated by an RNA recognition motif (RRM) contained in the Rna15 subunit of the complex. We show here that Rna15 has a strong and unexpected preference for GU containing RNAs and reveal the molecular basis for a base selectivity mechanism that accommodates G or U but discriminates against C and A bases. This mode of base selectivity is rather different to that observed in other RRM-RNA structures and is structurally conserved in CstF64, the mammalian counterpart of Rna15. Our observations provide evidence for a highly conserved mechanism of base recognition amongst the 3'-end processing complexes that interact with the U-rich or U/G-rich elements at 3'-end cleavage/polyadenylation sites.