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PDBsum entry 2rc5
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Oxidoreductase
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PDB id
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2rc5
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References listed in PDB file
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Key reference
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Title
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Crystal structures of leptospira interrogans FAD-Containing ferredoxin-Nadp+ reductase and its complex with NADP+.
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Authors
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A.S.Nascimento,
D.L.Catalano-Dupuy,
A.Bernardes,
M.D.E. .O.Neto,
M.A.Santos,
E.A.Ceccarelli,
I.Polikarpov.
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Ref.
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Bmc Struct Biol, 2007,
7,
69.
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PubMed id
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Abstract
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BACKGROUND: Ferredoxin-NADP(H) reductases (FNRs) are flavoenzymes that catalyze
the electron transfer between NADP(H) and the proteins ferredoxin or flavodoxin.
A number of structural features distinguish plant and bacterial FNRs, one of
which is the mode of the cofactor FAD binding. Leptospira interrogans is a
spirochaete parasitic bacterium capable of infecting humans and mammals in
general. Leptospira interrogans FNR (LepFNR) displays low sequence identity with
plant (34% with Zea mays) and bacterial (31% with Escherichia coli) FNRs.
However, LepFNR contains all consensus sequences that define the plastidic class
FNRs. RESULTS: The crystal structures of the FAD-containing LepFNR and the
complex of the enzyme with NADP+, were solved and compared to known FNRs. The
comparison reveals significant structural similarities of the enzyme with the
plastidic type FNRs and differences with the bacterial enzymes. Our small angle
X-ray scattering experiments show that LepFNR is a monomeric enzyme. Moreover,
our biochemical data demonstrate that the LepFNR has an enzymatic activity
similar to those reported for the plastidic enzymes and that is significantly
different from bacterial flavoenzymes, which display lower turnover rates.
CONCLUSION: LepFNR is the first plastidic type FNR found in bacteria and,
despite of its low sequence similarity with plastidic FNRs still displays high
catalytic turnover rates. The typical structural and biochemical characteristics
of plant FNRs unveiled for LepFNR support a notion of a putative lateral gene
transfer which presumably offers Leptospira interrogans evolutionary advantages.
The wealth of structural information about LepFNR provides a molecular basis for
advanced drugs developments against leptospirosis.
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