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PDBsum entry 2or1
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Gene regulation/DNA
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PDB id
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2or1
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Recognition of a DNA operator by the repressor of phage 434: a view at high resolution.
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Authors
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A.K.Aggarwal,
D.W.Rodgers,
M.Drottar,
M.Ptashne,
S.C.Harrison.
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Ref.
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Science, 1988,
242,
899-907.
[DOI no: ]
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PubMed id
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Abstract
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The repressors of temperate bacteriophages such as 434 and lambda control
transcription by binding to a set of DNA operator sites. The different affinity
of repressor for each of these sites ensures efficient regulation.
High-resolution x-ray crystallography was used to study the DNA-binding domain
of phage 434 repressor in complex with a synthetic DNA operator. The structure
shows recognition of the operator by direct interactions with base pairs in the
major groove, combined with the sequence-dependent ability of DNA to adopt the
required conformation on binding repressor. In particular, a network of
three-centered bifurcated hydrogen bonds among base pairs in the operator helps
explain why 434 repressor prefers certain sites over others. These bonds, which
stabilize the conformation of the bound DNA, can form only with certain
sequences.
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Secondary reference #1
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Title
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Structure of the repressor-Operator complex of bacteriophage 434.
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Authors
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J.E.Anderson,
M.Ptashne,
S.C.Harrison.
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Ref.
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Nature, 1987,
326,
846-852.
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PubMed id
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