PDBsum entry 2ex4

Transferase

PDB id: 2ex4

Contents

Protein chains

221 a.a. *

Ligands

SAH ×2

Waters ×555

* Residue conservation analysis

PDB id:

2ex4

Name:

Transferase

Title:

Crystal structure of human methyltransferase ad-003 in complex with s- adenosyl-l-homocysteine

Structure:

Adrenal gland protein ad-003. Chain: a, b. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. (Stratagene).

Resolution:

1.75Å R-factor: 0.197 R-free: 0.258

Authors:

J.R.Min,H.Wu,H.Zeng,P.Loppnau,M.Sundstrom,C.H.Arrowsmith,A.M.Edwards, A.Bochkarev,A.N.Plotnikov,Structural Genomics Consortium (Sgc)

Key ref:

J.Min et al. The crystal structure of human ad-003 protein in complex with s-Adenosyl-L-Homocysteine. To be published, .

Date:

07-Nov-05 Release date: 15-Nov-05

Protein chains

Q9BV86  (NTM1A_HUMAN) -  N-terminal Xaa-Pro-Lys N-methyltransferase 1 from Homo sapiens

Seq: 223 a.a.

Struc: 221 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.1.1.244 - protein N-terminal methyltransferase.
• Reaction:
  1. N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L- methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl- [protein] + 3 S-adenosyl-L-homocysteine + 3 H⁺
  2. N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L- methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl- [protein] + 3 S-adenosyl-L-homocysteine + 3 H⁺
  3. N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L- methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H⁺

N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 × S-adenosyl-L- methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl- [protein] + 3 × S-adenosyl-L-homocysteine + 3 × H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 × S-adenosyl-L- methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl- [protein] + 3 × S-adenosyl-L-homocysteine + 3 × H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 × S-adenosyl-L- methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 × S-adenosyl-L-homocysteine + 2 × H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site