PDBsum entry 2daa

Pyridoxal phosphate

PDB id: 2daa

Contents

Protein chains

277 a.a. *

Ligands

DCS ×2

Waters ×200

* Residue conservation analysis

PDB id:

2daa

Name:

Pyridoxal phosphate

Title:

Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine

Structure:

D-amino acid aminotransferase. Chain: a, b. Engineered: yes. Other_details: complexed with cycloserine pyridoxal-5'-phosphate

Source:

Bacillus sp.. Organism_taxid: 1409. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932. Other_details: thermophilic bacillus sp.

Biol. unit:

Dimer (from PDB file)

Resolution:

2.10Å R-factor: 0.205 R-free: 0.260

Authors:

D.Peisach,D.M.Chipman,D.Ringe

Key ref:

D.Peisach et al. (1998). D-Cycloserine inactivation of d-Amino acid aminotransferase leads to a stable noncovalent protein complex with an aromatic cycloserine-Plp derivative. J am chem soc, 120, 2268-2274.

Date:

27-Oct-97 Release date: 18-Mar-98

Protein chains

P19938  (DAAA_BACYM) -  D-alanine aminotransferase from Bacillus sp. (strain YM-1)

Seq: 283 a.a.

Struc: 277 a.a.

Enzyme reactions

• Enzyme class: E.C.2.6.1.21 - D-amino-acid transaminase.
• Reaction: D-alanine + 2-oxoglutarate = D-glutamate + pyruvate
• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = DCS) matches with 65.22% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site